Association Energetics of Cross-Reactive and Specific Antibodies

Association energetics of cross-reactive and specific antibodies.

HyHEL-8, HyHEL-10, and HyHEL-26 (HH8, HH10, and HH26, respectively) are murine monoclonal IgG(1) antibodies which share over 90% variable-region amino acid sequence identity and recognize identical structurally characterized epitopes on hen egg white lysozyme (HEL). Previous immunochemical and surface plasmon resonance-based studies have shown that these antibodies differ widely in their tolera...

Antibodies Antibodies in the Absence of Neutralizing Antibody-Dependent Cellular Cytotoxicity Cross-Reactive Influenza-Specific

Age-related prevalence of cross-reactive antibodies

B Blümel ([email protected])1,2,3,4, B Schweiger1, M Dehnert1,5, S Buda1, A Reuss1, I Czogiel1, P Kamtsiuris1, M Schlaud1, C Poethko-Müller1, M Thamm1, W Haas1 1. Robert Koch Institute, Berlin, Germany 2. Postgraduate Training for Applied Epidemiology (PAE, German FETP), Robert Koch-Institute, Berlin, Germany 3. European Programme for Intervention Epidemiology Training (EPIET), European...

Identification of species-specific and cross-reactive epitopes in human polyomavirus capsids using monoclonal antibodies.

The human antibody response to polyomavirus capsid proteins is not well characterized. Recombinant BK virus (BKV), JC virus (JCV) and simian virus 40 (SV40) virus-like particles (VLP) were produced in a baculovirus system, and mouse monoclonal antibodies (mAbs) to these proteins were generated using standard methods. Nine of 12 BKV mAbs showed neutralizing activity. The non-neutralizing antibod...

Characterization of stage-specific and cross-reactive antigens from Eimeria acervulina by chicken monoclonal antibodies.

The characterization of five chicken monoclonal antibodies (mAbs) that were developed against apical complex antigens of Eimeria acervulina sporozoites is realized and the mAbs reactivity to merozoites belonging to this species is tested. Using immuno-fluorescence assay (IFA), one mAb (HE-4) that recognized apical antigens common to sporozoites of E. acervulina and E. brunetti bound antigens lo...